2.4 Statistical analysis
All data was performed using GraphPad Prism (version 8.0.2) software for
statistical analysis and graphic presentations. The normality of the
data was tested by the Shapiro-Wilk test(n<50). Normally
distributed data was expressed as mean± standard deviation, and
non-normally distributed data was represented by the median (minimum
number-maximum number). The Mann-Whitney U-test was used for the data
without normal distribution. The T-test was used for normally
distributed paired samples.The correlation analysis between two
continuous variables that were non-normally distributed was analyzed
using Spearman’s rank correlation. The statistical significance was
determined as P <.05 (*P < .05,
**P < .01,****P < .0001).