2.4 Statistical analysis
All data was performed using GraphPad Prism (version 8.0.2) software for statistical analysis and graphic presentations. The normality of the data was tested by the Shapiro-Wilk test(n<50). Normally distributed data was expressed as mean± standard deviation, and non-normally distributed data was represented by the median (minimum number-maximum number). The Mann-Whitney U-test was used for the data without normal distribution. The T-test was used for normally distributed paired samples.The correlation analysis between two continuous variables that were non-normally distributed was analyzed using Spearman’s rank correlation. The statistical significance was determined as P <.05 (*P < .05, **P < .01,****P < .0001).