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Enhanced 2-phenylethanol production by newly isolated
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  • Fengxue Xin,
  • Wei Yan,
  • Xiujuan Qian,
  • Wenming Zhang,
  • Jie Zhou,
  • Weiliang Dong,
  • Bin Xu,
  • Min Jiang
Fengxue Xin
Nanjing University of Technology
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Wei Yan
Nanjing Tech University
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Xiujuan Qian
Nanjing Tech University
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Wenming Zhang
Nanjing University of Technology
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Jie Zhou
Nanjing Tech University
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Weiliang Dong
Nanjing University of Technology
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Bin Xu
Nanjing Tech University
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Min Jiang
Nanjing University of Technology
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Abstract

A unique Meyerozyma sp. strain YLG18 was obtained in this study, which was capable of producing 2-phenylethanol through both Ehrlich and Shikimate pathways. Response surface methodology (RSM) was implemented to improve the maximum 2-PE production. At optimized conditions: temperature, 24.7℃; initial glucose, 63.54 g/L; initial L-phe, 10.70 g/L, 2-PE production was increased to 2.55 g/L, leading to 104% increase compared to the pre-optimized one. In situ product recovery (ISPR) could further help improve the final 2-PE production to 3.20 g/L with fatty acid methyl ester as the extractant, representing the highest 2-PE production by using Meyerozyma sp.. Furthermore, genes involved in 2-PE synthesis were identified and their expression levels between Shikimate pathway and Ehrlich pathway were compared. Based on the genomic and transcriptional analysis, a penta-functional enzyme AroM in Shikimate pathway and an aspartate aminotransferase (AAT) with the potential to convert phenylalanine into phenylpyruvate in Ehrlich pathway were identified. These findings would help broaden our knowledge and add to the pool of known 2-PE generating microbes and genes.