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Antibodies specific to Ferritin, light polypeptide (FTL) are frequently detected in Immunorelated Pancytopenia patients
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  • Shanfeng Hao,
  • yang zhang,
  • luogang hua,
  • ning xie,
  • na xiao,
  • xiaojing wu,
  • huaquan wang ,
  • rong fu,
  • zonghong shao
Shanfeng Hao
Tianjin Medical University General Hospital
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yang zhang
Tianjin Medical University General Hospital
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luogang hua
Tianjin Medical University General Hospital
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ning xie
Tianjin Medical University General Hospital
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na xiao
Tianjin Medical University General Hospital
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xiaojing wu
Peking University BinHai Hospital
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huaquan wang
Tianjin Medical University General Hospital
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rong fu
Tianjin Medical University General Hospital
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zonghong shao
Tianjin Medical University General Hospital
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Abstract

Immuno-related pancytopenia (IRP) is featured by pancytopenia resulting from bone marrow suppression or destruction mediated by autoantibodies (auto-Abs). In a previous study we have established a K562 cDNA library, which was applied in screening 7 possible autoantigens produced by hematopoietic cells in IRP patients, including FTL. In this study FTL was expressed and purified, and then we detected the level of the auto-Abs specific to FTL. Through enzyme-linked immunosorbent assay (ELISA), it is revealed that the titer of anti–FTL antibodies (FTL Abs) was higher in IRP patients without treatment compared with those who recovered from IRP, those with severe aplastic anemia(SAA), those with myelodyplastic syndrome(MDS) and healthy controls. Furthermore, the level of FTL-mRNA was up-regulated in IRP patients without treatment compared with those who recovered from IRP, MDS patients and normal controls. These findings indicate that FTL Abs are highly expressed in IRP patients. Detecting FTL Abs might have some clinical value in differentiating IRP from SAA and MDS. Moreover, in certain type of IRP patients, FTL, as an autoantigen, may induce immune attack on hematopoietic stem cells.