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Olfactory receptor phylogeny reveals conserved channels for sex pheromone and host plant signals in tortricid moths
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  • Francisco Gonzalez,
  • Felipe Borrero-Echeverry,
  • Julia Josvai,
  • Maria Strandh,
  • Rikard Unelius,
  • Miklos Toth,
  • Peter Witzgall,
  • Marie Bengtsson,
  • William Walker
Francisco Gonzalez
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Felipe Borrero-Echeverry
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Julia Josvai
Plant Protection Institute CAR HAS, Budapest, Hungary
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Maria Strandh
Molecular Ecology and Evolution Lab, Dept. Biology, Lund University
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Rikard Unelius
Faculty of Health and Life Sciences, Linnaeus University, Kalmar
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Miklos Toth
Plant Protection Institute CAR HAS, Budapest, Hungary
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Peter Witzgall
Swedish University of Agricultural Sciences
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Marie Bengtsson
Swedish University of Agricultural Sciences
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William Walker
Swedish University of Agricultural Sciences
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Abstract

The search for mates and food is mediated by volatile chemicals. Insects sense food odorants and sex pheromones through odorant receptors (ORs) and pheromone receptors (PRs), which are expressed in olfactory sensory neurons. Investigating the receptive range of these receptors instructs the identification of behaviourally relevant chemicals. Studying orthologous receptors and their ligands across taxa affords insights into the role of chemical communication in reproductive isolation and phylogenetic divergence. The female sex pheromone of green budworm moth Hedya nubiferana (Lepidoptera, Totricidae) is a blend of two unsaturated acetates, only a blend of both elicits male attraction. Females also produce codlemone, which is the sex pheromone of another tortricid, codling moth Cydia pomonella. Codlemone also attracts green budworm moth males. Concomitantly, green budworm and codling moth males are attracted to the food plant volatile pear ester. A congruent behavioural response to the same pheromone and plant volatile in two tortricid species suggests co-occurrence of dedicated odorant receptors. In codling moth, one PR is tuned to both compounds, the sex pheromone codlemone and the plant volatile pear ester. Our phylogenetic analysis finds that green budworm moth expresses an orthologous PR gene. Shared ancestry, and high levels of amino acid identity and sequence similarity, in codling and green budworm moth PRs offers an explanation for parallel attraction of both species to the same compounds. A conserved olfactory channel for a sex pheromone and host plant volatile substantiates the alliance of social and habitat signals in insect chemical communication. Field attraction assays confirm that in silico investigations of odorant receptors afford powerful predictions for an efficient identification of behaviour-modifying semiochemicals, for an improved understanding of the mechanisms of host plant attraction in insect herbivores and for the further development of sustainable insect control.

Peer review status:ACCEPTED

14 Feb 2020Submitted to Ecology and Evolution
15 Feb 2020Submission Checks Completed
15 Feb 2020Assigned to Editor
19 Feb 2020Reviewer(s) Assigned
26 Mar 2020Review(s) Completed, Editorial Evaluation Pending
27 Mar 2020Editorial Decision: Revise Minor
08 May 20201st Revision Received
08 May 2020Submission Checks Completed
08 May 2020Assigned to Editor
08 May 2020Review(s) Completed, Editorial Evaluation Pending
12 May 2020Editorial Decision: Accept