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Immuno-Related Gene Polymorphisms associated with Acute Myeloid Leukemia
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  • Qinqin Liu,
  • Mingqiang Hua,
  • Shuxin Yan,
  • Chen Zhang,
  • Ruiqing Wang,
  • Xinyu Yang,
  • Fengjiao Han,
  • Ming Hou,
  • daoxin ma
Qinqin Liu
Shandong University
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Mingqiang Hua
Shandong University Qilu Hospital
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Shuxin Yan
Shandong University Qilu Hospital
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Chen Zhang
Shandong University Qilu Hospital
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Ruiqing Wang
Shandong University Qilu Hospital
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Xinyu Yang
Shandong University Qilu Hospital
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Fengjiao Han
Shandong University Qilu Hospital
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Ming Hou
Shandong University Qilu Hospital
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daoxin ma
Shandong University Qilu Hospital
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Abstract

Though the pathogenesis of acute myeloid leukemia (AML) is still unknown, accumulating evidence has revealed that immune response plays a vital part in the pathogenesis. Here, we investigated the involvement of 24 single-nucleotide polymorphisms (SNPs) of immuno-related genes, including cytokines (IL2, IL4, IL9, IL-12A, IL-22, IFNG, and TGFB1), transcriptional regulatory genes (TBX21, STAT1, STAT3, STAT5B, STAT6, GATA3, FOXP3, and IRF4), and others (IL2RA IL6R NFKBIA), in 269 AML inpatients and 200 healthy controls. Furthermore, we analyzed the relationship between the SNPs and clinical characteristics. Immuno-related SNP genotyping was performed on the Sequenom MassARRAY iPLEX platform. All the SNPs in healthy controls were consistent with Hardy–Weinberg equilibrium. All final p values were adjusted by Bonferroni multiple testing. Our results showed that IL-22 (rs2227491) was significantly associated with the white blood cell (WBC) counts. STAT5B (rs6503691) showed a close relationship with the recurrent genetic abnormalities in patients with AML. We verified the negatively independent effect of age and risk of cytogenetics on overall survival (OS). More importantly, the GG genotype of IL-12A (rs6887695) showed a negative impact on AML prognosis independently. Furthermore, the relative expression of IL-12 was decreased in GG genotype, no matter under codominant or recessive model. However, no correlation was observed between the SNPs mentioned above and disease susceptibility, risk stratification, and survival. Our findings suggest that immuno-related gene polymorphisms are associated with prognosis in AML, which may perform as novel inspection targets for AML patients.

Peer review status:ACCEPTED

17 Feb 2020Submitted to Clinical & Experimental Immunology
18 Feb 2020Submission Checks Completed
18 Feb 2020Assigned to Editor
10 Mar 2020Reviewer(s) Assigned
24 Mar 2020Review(s) Completed, Editorial Evaluation Pending
24 Mar 2020Editorial Decision: Revise Minor
09 Apr 20201st Revision Received
09 Apr 2020Review(s) Completed, Editorial Evaluation Pending
13 Apr 2020Editorial Decision: Revise Minor
14 Apr 20202nd Revision Received
15 Apr 2020Review(s) Completed, Editorial Evaluation Pending
23 Apr 2020Editorial Decision: Accept