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Alveolar mimics with periodic strain and its effect on the cell layer formation
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  • Milad Radiom,
  • Yong He,
  • Juan Peng,
  • Armelle Baeza-Squiban,
  • Jean-Francois Berret,
  • Yong Chen
Milad Radiom
Universite de Paris
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Yong He
École Normale Supérieure
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Juan Peng
École Normale Supérieure
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Armelle Baeza-Squiban
Universite de Paris
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Jean-Francois Berret
Universite de Paris
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Yong Chen
Ecole Normale Superieure
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Abstract

We report on the development of a new model of alveolar air-tissue interface consisting of an array of suspended hexagonal monolayers of gelatin nanofibers supported by microframes and a microfluidic device for the patch integration. The suspended monolayers are deformed to a central displacement of 40-80 μm at the air-liquid interface by application of air pressure in the range of 200-1000 Pa. With respect to the diameter of the monolayers that is 500 μm, this displacement corresponds to a linear strain of 2-10% in agreement with the physiological strain range in the lung alveoli. The culture of A549 cells on the monolayers for an incubation time 1-3 days showed viability in the model. We exerted a periodic strain of 5% at a frequency of 0.2 Hz during 1 hour to the cells. We found that the cells were strongly coupled to the nanofibers, but the strain reduced the coupling and induced remodeling of the actin cytoskeleton, which led to a better tissue formation. Our model can serve as a versatile tool in lung investigations such as in inhalation toxicology and therapy.

Peer review status:ACCEPTED

17 Feb 2020Submitted to Biotechnology and Bioengineering
18 Feb 2020Submission Checks Completed
18 Feb 2020Assigned to Editor
24 Feb 2020Reviewer(s) Assigned
20 Mar 2020Review(s) Completed, Editorial Evaluation Pending
20 Mar 2020Editorial Decision: Revise Major
09 Apr 20201st Revision Received
10 Apr 2020Submission Checks Completed
10 Apr 2020Assigned to Editor
31 May 2020Reviewer(s) Assigned
13 Jun 2020Review(s) Completed, Editorial Evaluation Pending
13 Jun 2020Editorial Decision: Accept