From graphite to laccase biofunctionalized few-layer graphene: a “one
pot” approach using a chimeric enzyme
- Ilaria Sorrentino,
- Ilaria Stanzione,
- Yannig Nedellec,
- Alessandra Piscitelli,
- Paola Giardina,
- Alan le goff
Ilaria Stanzione
Universita degli Studi di Napoli Federico II Complesso Universitario di Monte Sant\'Angelo
Author ProfileAlessandra Piscitelli
Universita degli Studi di Napoli Federico II Complesso Universitario di Monte Sant\'Angelo
Author ProfileAbstract
A chimeric enzyme based on the genetic fusion of a laccase with a
hydrophobin domain was employed to functionalize few-layer-graphene,
previously exfoliated from graphite in the presence of the hydrophobin.
The as-produced biofunctionalized FLG was characterized by
electrochemistry and Raman spectroscopy and finally employed in the
biosensing of phenols such as catechol and dopamine. This strategy paves
the way for the functionalization of nanomaterials by hydrophobin
domains of chimeric enzymes and their use in a variety of
electrochemical applications.