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Cell-derived Extracellular Matrix - a promising Biomaterial for in vitro-vascularization in Adipose Tissue Engineering
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  • Svenja Nellinger,
  • Isabelle Schmidt,
  • Simon Heine,
  • Ann-Cathrin Volz,
  • Petra Kluger
Svenja Nellinger
Reutlingen University
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Isabelle Schmidt
Reutlingen University
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Simon Heine
Reutlingen University
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Ann-Cathrin Volz
Reutlingen University
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Petra Kluger
Reutlingen University
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Abstract

Tissue constructs of physiologically relevant scale require a vascular system to maintain cell viability. However, in vitro vascularization of engineered tissues is still a major challenge. Successful approaches are based on a feeder layer (FL) to support vascularization. Here, we investigated whether the supporting effect on the self-assembled formation of vascular-like structures by microvascular endothelial cells (mvECs) originates from the FL itself or from its extracellular matrix (ECM). Therefore, we compared the influence of ECM, either derived from adipose-derived stem cells (ASCs) or adipogenic differentiated ASCs, with the classical approaches based on a cellular FL. All cell-derived ECM (cdECM) substrates enable mvEC growth with high viability. Vascular-like structure formation was visualized by immunofluorescence staining of endothelial surface protein CD 31 and can be observed on all cdECM and FL substrates but not on control substrate collagen I. On adipogenic differentiated ECM longer and higher branched structures can be found compared to stem cell cdECM. An increased concentration of pro-angiogenic factors can be found in cdECM substrates and FL approaches compared to controls. Finally, expression of proteins associated with tube formation (E-selectin and thrombomodulin) was confirmed. These results highlight cdECM as promising biomaterial for in vitro vascularization in adipose tissue engineering.

Peer review status:ACCEPTED

20 Feb 2020Submitted to Biotechnology and Bioengineering
21 Feb 2020Submission Checks Completed
21 Feb 2020Assigned to Editor
24 Feb 2020Reviewer(s) Assigned
09 Apr 2020Review(s) Completed, Editorial Evaluation Pending
09 Apr 2020Editorial Decision: Revise Major
24 May 20201st Revision Received
26 May 2020Submission Checks Completed
26 May 2020Assigned to Editor
31 May 2020Reviewer(s) Assigned
24 Jun 2020Review(s) Completed, Editorial Evaluation Pending
24 Jun 2020Editorial Decision: Accept