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Development of second generation ethanol process based on xylose consumption using non-conventional and recombinant yeasts
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  • Isabela Pereira,
  • Ângela dos Santos,
  • Davi Gonçalves,
  • Marcela Purificação,
  • Nick Guimarães,
  • Robson Tramontina,
  • Natalia Coutouné,
  • Eduardo Zanella,
  • Boris Stambuk ,
  • Jaciane Ienczak
Isabela Pereira
Universidade Federal de Santa Catarina Centro Tecnologico
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Ângela dos Santos
Federal University of Santa Catarina
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Davi Gonçalves
Federal University of Santa Catarina
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Marcela Purificação
Federal University of Santa Catarina
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Nick Guimarães
Universidade Federal de Santa Catarina Centro Tecnologico
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Robson Tramontina
Universidade Estadual de Campinas
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Natalia Coutouné
National Center for Research in Energy and Materials (CNPEM)
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Eduardo Zanella
Federal University of Santa Catarina
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Boris Stambuk
Federal University of Santa Catarina
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Jaciane Ienczak
Universidade Federal de Santa Catarina
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Abstract

Fermentation of the pentose fraction from hemicellulosic hydrolysate is an important challenge to be studied in second generation ethanol (E2G) production. In this sense, we have tested non-conventional (Scheffersomyces stipitis and Spathaspora passalidarum) and recombinant yeast strains (Saccharomyces cerevisiae recombinant MP-C5 and MP-C5H1) capable to uptake xylose with the aim to design a strategy for E2G production. Growth tests in different carbohydrates (glucose, xylose, glucose + xylose and sucrose + xylose) have shown that the integration of xylose and sucrose presented better results for all yeast tested, and the co-fermentation of these sugars provided faster xylose consumption by S. cerevisiae recombinant. One the other hand, Sp. passalidarum do not present high performance of sucrose consumption in microanaerobic fermentation conditions and an intracellular invertase high activity was observed by this yeast. S. cerevisiae MP-C5H1 presented best performance for fermentation tests and a batch strategy with high cell density was designed. By this strategy was possible to achieve high ethanol yield (0.48 g g-1), titer (53.7 g L-1) and global ethanol productivity (2.24 g L-1 h-1).

Peer review status:UNDER REVIEW

02 Mar 2020Submitted to Biotechnology and Bioengineering
02 Mar 2020Assigned to Editor
02 Mar 2020Submission Checks Completed
17 Mar 2020Reviewer(s) Assigned
04 May 2020Review(s) Completed, Editorial Evaluation Pending