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The E-2-hexenal play critical role in carotenoids activated inner membrane structures transformation in the chloroplasts
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  • Xince Wang,
  • Fei Sun,
  • Cong Han,
  • Qingmin Chen,
  • Xiaoying Yang,
  • Jianfeng Cheng,
  • Lili Zhu,
  • Jingjing Liu,
  • Feifei Tan,
  • Maorun Fu,
  • Bo Cui
Xince Wang
Qilu University of Technology
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Fei Sun
Qilu University of Technology
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Cong Han
Qilu University of Technology
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Qingmin Chen
Shandong Agriculture and Engineering University
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Xiaoying Yang
Qilu University of Technology
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Jianfeng Cheng
Jiangxi Agricultural University
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Lili Zhu
Jiangxi Agricultural University
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Jingjing Liu
Jiangxi Agricultural University
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Feifei Tan
Beijing Science and Technology Publishing Co Ltd
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Maorun Fu
Qilu University of Technology
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Bo Cui
Qilu University of Technology
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Abstract

The carotenoids start the inner membrane structures (IMS) transformation of chloroplasts during colour transformation in tomatoes, but the mechanism remains unclear. E-2-hexenal (E2H) may function downstream of carotenoids to regulate the transformation. In the tomatoes treated with E2H, colour transformation was inhibited and the colour transformation cannot be initiated by ethylene (ET) again, whereas in the ET pretreated tomatoes, the E2H lost the function of inhibition. Therefore, the E2H function downstream of ET. The electron micrographs showed the exogenous E2H accelerated the IMS transformation before enough carotenoids accumulated. Therefore, the E2H may acted downstream of the carotenoids starting the IMS transformation. The contents and the biosynthesis locations of chlorophyll, lycopene and β-carotene supported the E2H accelerated IMS transformation. The E2H expedited LOXC expression caused advanced free linolenic acids consume, E2H also accelerate the release of linolenic acids directly, thus the composition changes altered the IMS. The HPL expression patterns may related with the endogenous E2H feedback balance. The titratable acids, ascorbic acids, soluble solids and malondialdehyde contents also supported the hypothesis. Above all, E2H mediated the carotenoids started IMS transformation. Novel function of the E2H was proposed. Keywords: tomato, E-2-hexenal, chloroplasts, chromoplast, plastids, colour, carotenoid