Differential effects elicited by neocuproine, a copper (1) chelator, on
the bladder activity in mouse and rat
Abstract
Background and Purpose: The aim of this study was to investigate the
mechanism of a possible differential effect of Neocuproine (NC),
selective Cu(1) chelator, in rat and mouse bladder tissues. Experimental
Approach: Bladder function was evaluated by 1. in vitro isolated bladder
strips, 2. in vitro preparations of whole bladders and 3. İn vivo
cystometrogram (CMG) in rat and mouse. Selective Cu(I) and Cu(II)
chelators and non-selective purinergic antagonists were examined on
EFS-induced bladder contractions in isolated bladder strips. The
spontaneous contraction activities of whole bladders were recorded to
evaluate the amplitudes and frequencies. In CMGs experiments, the values
of maximum bladder pressure during micturition and intercontraction
intervals (ICI) were evaluated. Key Results: Whereas Neocuproine (NC)
enhances the opposite effect on isolated bladder strips and whole
bladder experiments, this opposite effect was not observed in in vivo
CMG experiments in rat and mouse. NC caused a significant suppression on
spontaneous contractions and baseline tonus in isolated mouse bladder
tissues, whereas it caused facilitating effects in the rat. However, NC
significantly decreased the ICI in both rat and mouse in CMGs.
Conclusion and Implications: The effect of Neocuproine on rat and mouse
bladder activity is likely to be the role of myogenic mechanisms. It is
possible to trigger mechanisms associated with intracellular calcium
(Ca2+) reduction and purinergic pathway, P2X purinoceptors besides P2Y
purinoceptors, may have an important role in the inhibitory activity of
NC on mouse bladder.