Increase of the lethal activity of Cytotoxic T cells and Natural killer
cells as a result of combination between Con-A and IL-2 in the initial
stages from the treatment of lung cancer patients
Abstract
Cytotoxic T cells are one of the most specialized immune cells to defend
the body against foreign invaders in the best scenario, but failure in
the function of these cells, leading to decrease defense of the body
against cancer. We aims to address the functionality of cytotoxic T
lymphocytes (CTLs), Natural killer (NK) and Natural killer T-cells (NKT)
cells in lung cancer patient’s pre and post-stimulation of these cells
in-vitro using laboratory activation protocol. Five healthy persons as
volunteers and fifteen lung cancer patients were subjected to this
study. The count of CTLs, NK and NKT cells and the expression of their
intracellular granzyme B (GzB) pre and post-stimulation for 72 hrs
in-vitro was determined. The plasma level of cytokines and chemokines
was correlated with the patient’s prognosis. Data showed that after
culture, there were highly significantly increased in the fold change of
the percentage of GzB expression on CD3, CD4, CD8 and NKCD8 T cells in
lung cancer patients before induction of chemotherapy when compared to
healthy control and other lung cancer patients. The level of
Proinflammatory cytokines in patients before and during induction of
chemotherapy showed IL-1 and CCL-2 in a 13000, 250 fold increase
respectively while it decreases in patients after induction of
chemotherapy compared with the control group. Patients before the
induction of chemotherapy have the ability to improve their CTL and NK
functionality under activation conditions. The use of immune activation
mechanisms is needed before the induction of chemotherapy.