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A Novel Water-Soluble Photosensitizer for Photodynamic Inactivation of Gram-Positive Bacteria
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  • Zihuayuan Yang,
  • Ying Qiao,
  • Junying Li,
  • Fu-Gen Wu,
  • Fengming Lin
Zihuayuan Yang
Southeast University
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Ying Qiao
Southeast University
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Junying Li
Southeast University
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Fu-Gen Wu
Southeast University
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Fengming Lin
Southeast University
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Abstract

Antimicrobial photodynamic therapy (APDT) is a promising alternative to traditional antibiotics for bacterial infections, which inactivates a broad spectrum of bacteria. However, it has some disadvantages including poor water solubility and easy aggregation of hydrophobic photosensitizers (PS), and poor tissue penetration and cytotoxicity when using UV as light source, leading to photodynamic therapy efficacy. Herein, we develop a novel water-soluble natural PS (sorbicillinoids) obtained by microbial fermentation using filamentous fungus Trichoderma reesei (T. reesei). Sorbicillinoids could effectively generate singlet oxygen (1O2) under UV light irradiation, and ultimately display photoinactivation activity on Gram-positive bacteria, but not Gram-negative ones. Staphylococcus aureus (S. aureus) treated with sorbicillinoids and UV light displayed high levels of intracellular reactive oxygen species (ROS), notable DNA photocleavage, and compromised membrane permeability without overt cell membrane disruption. Moreover, the dark toxicity, phototoxicity or hemolysis activity of sorbicillinoids is negligible, showing its excellent biocompatibility.

Peer review status:UNDER REVIEW

09 Apr 2020Submitted to Biotechnology and Bioengineering
10 Apr 2020Assigned to Editor
10 Apr 2020Submission Checks Completed
13 Apr 2020Reviewer(s) Assigned
26 Apr 2020Review(s) Completed, Editorial Evaluation Pending