A Cross-Sectional Study on Sinonasal Inverted Papilloma: Does Human
Papilloma Virus Play a Role in Its Etiology?
Aims: To correlate the HPV genotypes with recurrence of disease and
malignant transformation. Methods: A prevalence cross-sectional study.
The tumour tissue was isolated from the paraffin-embedded tissue (PET).
The DNA was extracted from the tissue using the QiAamp DNA Mini Kit
(Qiagen, Germany). Gel electrophoresis was performed to determine the
presence of genomic DNA. HPV detection and genotyping were carried out
using SACACE HPV High Risk and Low Risk Typing Real-TM kit (SACACE,
Italy). Two different types of kits were used, that is HPV 6,11 Real-TM
and HPV 16,18 Real-TM kits. Results: A total of 44 patients, 36 were
male and 8 were female with a ratio of 5:1. 61.4% was Malay, 22.7% was
Chinese, 11.4% Indian 4.5% other races. 15 out of 44 patients had HPV
positive (34%). The recurrence rate of positive HPV infection compared
to negative HPV was not statistically significant (p>0.05).
There was a significant correlation of HPV 16 and 18 infection with
malignant transformation (p<0.05). A high detection rate of a
high-risk HPV type (67%) was observed in patients with inverted
papilloma with malignant transformation. Conclusion: The prevalence of
HPV in inverted papilloma is 34%. Our result supports that HPV
infection is an aetiological factor in sinonasal inverted papilloma. A
high-risk HPV plays a role in the oncogenesis of sinonasal inverted
papilloma. Further studies should be conducted to further elaborate this