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Enhanced microbial corrosion by Acidithiobacillus ferrooxidans through the manipulation of substrate oxidation and genetic engineering
  • Yuta Inaba,
  • Alan West,
  • Scott Banta
Yuta Inaba
Columbia University
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Alan West
Columbia University
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Scott Banta
Columbia University
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Abstract

Acidithiobacillus ferrooxidans cells can oxidize iron and sulfur and are key members of the microbial biomining communities that are exploited in the large-scale bioleaching of metal sulfide ores. Some minerals are recalcitrant to bioleaching due to presence of other inhibitory materials in the ore bodies. Additives are intentionally included in processed metals to reduce environmental and microbially influenced corrosion. We have previously reported a new aerobic corrosion mechanism where A. ferrooxidans cells combined with pyrite and chloride can oxidize low grade stainless steel (SS304) with a thiosulfate-mediated mechanism. Here we explore process conditions and genetic engineering of the cells to enable corrosion of a higher grade steel (SS316). The addition of elemental sulfur and an increase in the cell loading resulted in a 74% increase in the corrosion of SS316 as compared to sulfur- and cell-free control experiments. The overexpression of the endogenous rus gene, which is involved in the cellular iron oxidation pathway, led to further 85% increase in the corrosion of the steel. Thus, the modification of the culturing conditions and cell line, led to a more than 3-fold increase in the corrosion of SS316 stainless steel, such that 15% of the metal coupons was dissolved in just 2 weeks. This work demonstrates how the engineering of cells and the optimization of their cultivation conditions can be used to discover conditions that lead to the corrosion of a complex metal target.

Peer review status:ACCEPTED

16 Apr 2020Submitted to Biotechnology and Bioengineering
17 Apr 2020Submission Checks Completed
17 Apr 2020Assigned to Editor
23 Apr 2020Reviewer(s) Assigned
29 May 2020Review(s) Completed, Editorial Evaluation Pending
29 May 2020Editorial Decision: Revise Major
24 Jun 20201st Revision Received
25 Jun 2020Submission Checks Completed
25 Jun 2020Assigned to Editor
10 Jul 2020Review(s) Completed, Editorial Evaluation Pending
10 Jul 2020Editorial Decision: Accept