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Transcriptional, metabolic, physiological and developmental responses of switchgrass to phosphorus limitation.
  • +2
  • Na Ding,
  • Raul Huertas,
  • Wei Liu,
  • Wolf-Ruediger Scheible,
  • Michael K. Udvardi
Na Ding
Noble Research Institute LLC
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Raul Huertas
Noble Research Institute LLC
Author Profile
Wei Liu
Noble Research Institute LLC
Author Profile
Wolf-Ruediger Scheible
Noble Research Institute LLC
Author Profile
Michael K. Udvardi
Noble Research Institute LLC
Author Profile

Abstract

Knowing how switchgrass (Panicum virgatum L.) responds and adapts to phosphorus (P)-limitation will aid efforts to optimize P-efficiency in this species for sustainable biomass production. This integrative study investigated the impacts of mild, moderate, and severe P-stress on genome transcription and whole-plant metabolism, physiology and development in an unprecedented way for this species. P-limitation reduced overall plant growth, increased root/shoot ratio, increased root branching at moderate P-stress, and decreased root diameter with increased density and length of root hairs at severe P-stress. RNA-seq analysis revealed thousands of genes that were differentially expressed under moderate and severe P-stress in roots and/or shoots compared to P-replete plants, with many stress-induced genes involved in transcriptional and other forms of regulation, primary and secondary metabolism, transport, and other processes involved in P-acquisition and homeostasis. Amongst the latter were multiple miRNA399 genes and putative targets of these. Metabolite profiling showed that levels of most sugars and sugar alcohols decreased with increasing P stress, while organic and amino acids increased under mild and moderate P-stress in shoots and roots, although this trend reversed under severe P-stress, especially in shoots.

Peer review status:UNDER REVIEW

16 Apr 2020Submitted to Plant, Cell & Environment
17 Apr 2020Submission Checks Completed
17 Apr 2020Assigned to Editor
24 Apr 2020Reviewer(s) Assigned
28 May 2020Review(s) Completed, Editorial Evaluation Pending
07 Aug 20201st Revision Received
11 Aug 2020Submission Checks Completed
11 Aug 2020Assigned to Editor
17 Aug 2020Review(s) Completed, Editorial Evaluation Pending