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Rapid intraoperative method for the identification of metastatic lymph nodes from thyroid carcinoma
  • +7
  • longshun shi,
  • Minhao Xie,
  • Guo Wu,
  • Jun Fan,
  • Mingming Guo,
  • Runlin Yang,
  • Jue Zhang,
  • Yi Zhang,
  • Bin Zhou,
  • Zhongwei Lv
longshun shi
NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine

Corresponding Author:[email protected]

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Minhao Xie
NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine
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Guo Wu
Hubei University of Medicine Affiliated Dongfeng Hospital
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Jun Fan
NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine
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Mingming Guo
NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine
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Runlin Yang
NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine
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Jue Zhang
Jiangsu Institution of Nuclear Medicine
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Yi Zhang
Jiangsu Institution of Nuclear Medicine
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Bin Zhou
Jiangsu Institution of Nuclear Medicine
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Zhongwei Lv
Shanghai 10th People’s Hospital, Tongji University School of Medicine, Shanghai, China
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Abstract

In this study, we established a rapid method for intraoperative identification of thyroid carcinoma metastatic lymph nodes. We developed a rapid method of thyroid globulin (Tg) detection called Tg-POCT based on time-resolved fluorescence immunoassay and validated reagent performance. Thyroid and lymph node tissues with metastatic thyroid cancer were considered as the positive group, and muscle, fiber, fat, and other tissues were considered as the negative control group to verify the above method combined with fine needle puncture. CV of the same batch number was 8.38% and that of different batch numbers was 11.24%. The minimum detection limit was 0.02 ng/mL. The test conformity of tissue samples was 100% by using the self-designed reference value. Thus, Tg-POCT is feasible as a rapid intraoperative method to identify thyroid cancer metastatic lymph nodes.