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Characteristics and epidemiology of extended-spectrum β-lactamase-producing Multidrug-Resistant Klebsiella pneumoniae from Red Kangaroo,China
  • +11
  • Xue Wang,
  • Qian Kang,
  • Jianan Zhao,
  • Zhihui Liu,
  • Fang Ji,
  • Junbao Li,
  • Jianchun Yang,
  • Chenglin Zhang,
  • Ting Jia,
  • Guoying Dong,
  • Shelan Liu,
  • Guocheng Hu,
  • Jianhua Qin,
  • Chengmin Wang
Xue Wang
Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public, Laboratory of Wild Animal Conservation and Utilization
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Qian Kang
Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public, Laboratory of Wild Animal Conservation and Utilization
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Jianan Zhao
Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public, Laboratory of Wild Animal Conservation and Utilization
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Zhihui Liu
Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public, Laboratory of Wild Animal Conservation and Utilization
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Fang Ji
Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public, Laboratory of Wild Animal Conservation and Utilization
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Junbao Li
Zhengzhou zoo
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Jianchun Yang
Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public, Laboratory of Wild Animal Conservation and Utilization
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Chenglin Zhang
Beijing Zoo
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Ting Jia
Beijing Zoo
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Guoying Dong
Beijing Normal University
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Shelan Liu
Zhejiang Provincial Center for Disease Control and Prevention
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Guocheng Hu
South China Institute of Environmental Sciences
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Jianhua Qin
Hebei Agricultural University
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Chengmin Wang
Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public, Laboratory of Wild Animal Conservation and Utilization
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Abstract

β-lactamase represents a serious challenge for treatment and public health, extended-spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae clones have been increasingly reported worldwide.However, little is known about the prevalence and biological characteristics of drug-resistant strains in zoos.During a routine surveillance at in Zhengzhou zoo of China, we reported firstly that the Klebsiella pneumoniae isolate from healthy Red Kangaroos (Macropus Rufus) showed severe MDR, especially resistant to Cefuroxime Sodium (MIC, >64 μg/ml), Ceftriaxone (MIC, >8 μg/ml) and Cefepime (MIC, >64 μg/ml) and belonged to ST290. The whole genome sequencing showed that Chrome Chr-M297-1 harbored blaDHA-3, blaSHV-1, blaCTX-M-14, FosA5, dfrA3, sul3 etc., pM297-1.1(222,864bp, IncFIB(K)) carried 9 antimicrobial genes including blaCTX-M-14 , blaTEM-191, APH(3’‘)-Ib, APH(6)-Id and QnrS1 etc., and pM297-1.2 (225,763bp, IncFII(K)) carried 22 antimicrobial genes including blaTEM-1, blaCTX-M-3, APH(3’)-Ia, AAC(3)-IIa, AAC(6’)-Ib-cr, aadA16, QnrB2, QnrS1, QacEΔ1, mphA, sul1, dfrA27, etc. Traceability analysis revealed that these two plasmids are highly similar to those recovered from human clinical samples in some southern cities in Sichuan Province, China(> 99%), suggesting the spread and distribution of these plasmids in China. Furthermore, two plasmids harboring conjugal transfer genes facilitate the transmission of antimicrobial genes by conjugation with E.coli J53. Our research shows that the transmission and adaptation of Klebsiella pneumoniae producing ESBLs in zoo environment, suggesting that the zoo is gradually becoming an important potential reservoir of clinically important drug-resistant genes.Therefore, it is necessary to strengthen the monitoring of the emergence and spread of drug-resistant strains in zoo environment in captive wild animals.