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Starch-based hydrogels show relevant properties for tissue engineering and loading of nanoparticulate systems.
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  • Seidy Pedroso-Santana,
  • Brian Rivas,
  • Noralvis Fleitas-Salazar,
  • Rafael Maura,
  • Carolina Gomez-Gaete,
  • Alexis Debut,
  • Natalie Parra,
  • Karla Vizuete,
  • Thelvia I. Ramos,
  • Jorge Toledo
Seidy Pedroso-Santana
Universidad de Concepcion
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Brian Rivas
Universidad de Concepcion
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Noralvis Fleitas-Salazar
Universidad de Concepcion
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Rafael Maura
Universidad de Concepcion
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Carolina Gomez-Gaete
Universidad de Concepcion
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Alexis Debut
Universidad de las Fuerzas Armadas
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Natalie Parra
Universidad de Concepcion
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Karla Vizuete
Universidad de las Fuerzas Armadas
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Thelvia I. Ramos
Universidad de las Fuerzas Armadas
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Jorge Toledo
Universidad de Concepcion
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Abstract

The synthesis of starch-based physical hydrogels in combination with chitosan and polyvinyl alcohol, and their potential co-application with chitosan nanoparticles was evaluated. The potential of starch-chitosan hydrogel obtained by physical/chemical method for tissue engineering uses was also studied in a mouse wound healing model. Although the microscopical structure of each synthesized hydrogel suggests a possible biological application, starch-polyvinyl alcohol hydrogel exhibited rigid behavior with minor channel diameters, a lower swelling rate (less than 300%), and negatively affected cell viability in a cytotoxicity assay. Starch-chitosan hydrogel obtained by chemical crosslinking with glutaraldehyde demonstrated the higher swelling rate (about 1100%), cell viability values over 80%, and a homogeneous tri-dimensional structure; along with an excellent interaction with chitosan nanoparticles. This type of hydrogel was selected for an in vivo experiment, showing significant differences in wound healing process against a non-treated control, in terms of inflammation, exudate production and tissue recovering.

Peer review status:POSTED

09 May 2020Submitted to Biotechnology and Bioengineering
11 May 2020Assigned to Editor
11 May 2020Submission Checks Completed