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Molecular characterization of clinical isolates from vascular access infection: A single institution study
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  • Yuan-Hsi Tseng,
  • Min Yi Wong,
  • Tsung-Yu Huang,
  • Bor-Shyh Lin,
  • Chun-Wu Tung,
  • Yao-Kuang Huang
Yuan-Hsi Tseng
Chiayi Chang Gung Memorial Hospital
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Min Yi Wong
Chiayi Chang Gung Memorial Hospital
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Tsung-Yu Huang
Chiayi Chang Gung Memorial Hospital
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Bor-Shyh Lin
National Chiao Tung University College of Photonics
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Chun-Wu Tung
Chiayi Chang Gung Memorial Hospital
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Yao-Kuang Huang
Chiayi Chang Gung Memorial Hospital
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Abstract

Hemodialysis requires repeated, reliable access to the systemic circulation; therefore, a well-functioning vascular access (VA) procedure is crucial for stable hemodialysis. VA infections (VAIs) constitute the most challenging complication and cause considerable morbidity, loss of access, and even death. In this study, we investigated the molecular profiles of different bacterial isolates retrieved from various types of VA grafts. We collected clinical isolates from hemodialysis patients with VAIs in our institution for the period between 2013 and 2018. We identified the bacterial isolates using standard biochemical procedures; we used a polymerase chain reaction for coagulase-negative staphylococcus (CoNS) and Burkholderia cepacia complex (BCC) species identification. The antibiotic resistance and molecular profile were analyzed using the disk diffusion method and multilocus sequence typing, respectively. We studied 150 isolates retrieved from patients with VAI and observed that Staphylococcus aureus was the predominant bacterial species, followed by S. argenteus, BCC, and CoNS. According to multilocus sequence typing data, we identified a wide variety of sequence types (STs) in S. aureus isolates, with ST59, ST45, and ST239 being the predominant types. Burkholderia cepacia with two new ST types, namely ST1723 and ST1724, accounted for most of the BCC infections, along with ST102 B. contaminans, which were mainly isolated from infected tunneled-cuffed catheters. In summary, the increased incidence of S. argenteus and BCC infections provides insights into their potential clinical effects in VAIs. The various STs identified in different bacterial species indicate the high genetic diversity of bacterial species isolated from VAIs in our institution.

Peer review status:IN REVISION

12 May 2020Submitted to MicrobiologyOpen
13 May 2020Assigned to Editor
13 May 2020Submission Checks Completed
15 May 2020Reviewer(s) Assigned
08 Jun 2020Review(s) Completed, Editorial Evaluation Pending
13 Jun 2020Editorial Decision: Revise Minor