Genome-Wide Identification of Grapevine's C3H2C3 Type RING E3 Ubiquitin
Ligases Family Reveals VyRCHC114, which Confers Tolerance to Drought
Stresses
Abstract
RING is one of the largest E3 ubiquitin ligase families, playing an
important role in plants’ development and growth and their biotic and
abiotic stress responses. The C3H2C3 type is the largest subfamily of
RING. Here, 143 C3H2C3-type RING genes (RCHCs) were discovered from the
grapevine genome and separated into groups (I~XI)
according to their phylogenetic analysis, with these genes named
according to their positions on chromosomes. Gene replication analysis
showed that 26 group segmental duplications and 14 tandem duplications
of VyRCHCs were identified. All VyRCHCs have introns, VyRCHC29 has a
maximum of 19 introns and 96 VyRCHCs have introns of 1 to 3. ARE, ABRE
and O2 site Cis-acting elements very conservative in the promoter of
VyRCHCs, with a large number. The expression profiles of eight DEGs in
RNA-Seq after drought stress were similar to those in qRT-PCR analysis.
The in vitro ubiquitin experiment showed that VyRCHC114 had E3 ubiquitin
ligase activity, while Arabidopsis thaliana overexpressing VyRCHC114 had
stronger tolerance of drought stress than the control. Valuable new
information on the evolution of grapevine RCHC family genes and its
relevance for studying the functional characteristics of grapevine
VyRCHC114 genes under drought stress emerged from this research.