Vitamin D and diacylglycerol reduce fat deposit and change liver lipid
metabolism and caecal microflora in rats fed a high-fat diet
Abstract
Vitamin D (VD) is associated with the release of leptin, which promotes
fat mobilization in the body. Diacylglycerol can reduce triacylglycerol
formation. This study evaluated the effects of administration of VD and
duck oil diacylglycerol on blood lipids, liver lipid metabolism and gut
microflora in rats fed a high-fat diet. A total of 75 rats were
allocated randomly to five treatments: non-obesity (NO), obesity control
(OC), diacylglycerol:10 mL per kg body weight (DG10), VD:12.5 μg per kg
body weight (VD12.5), and diacylglycerol + VD: 10 mL/d + 12.5 μg per kg
body weight (DG10VD12.5). Rats in NO group was fed the diet containing
7% soybean oil, while the other four groups were fed a high-fat (3.3%
soybean oil and 30.1% lard). VD and diacylglycerol were orally dosed
once per day. The administration of VD+diacylglycerol significantly
reduced the body weight gain and fat content. The change of fat deposit
was accompanied with the reduction in serum lipids, the increase in the
activity of enzymes involved in lipid metabolism in the liver, the
suppression of fatty acid synthase and the enhance of carnitine fatty
acyl transferase Ⅰ (CPT1) expression. The administration of VD and
diacylglycerol also significant modulated the bacterial diversity
profile in the cecum towards that in non-obesity rats. Those results
suggest that the administration of VD and diacylglycerol could have
significant roles in reducing fat deposit and altering gut microflora in
rats fed the high-fat diet.