Structural and Dynamic Analysis of Phyllosphere Fungal Community of
Walnut Leaves Infected by Leaf Spot Disease based Illumina
High-Throughput Sequencing Technology
Abstract
High-throughput sequencing technology was used to analyse the species
diversity, richness and dynamics of fungal communities in healthy and
infected walnut leaves. The dominant phyla included Ascomycota,
Basidiomycota, and Glomeromycota; the dominant classes were
Tremellomycetes, Dothideomycetes, and Leotiomycetes; the dominant orders
were Tremelellas, Capnodiales, and Erysiphales; the dominant families
were Bulleribasidiaceae, Mycosphaerellaceae, and Erysiphaceae; the
dominant genera were Vishniacozyma, Cercospora, and Ramularia; and the
dominant species were Vishniacozyma heimaeyensis, Cercospora asparagi,
and Cladosporium chasmanthicola. The results of this study also provided
a new understanding of the succession of the walnut phyllosphere fungal
community, filling a void in the knowledge of the microbial diversity
associated with walnut leaf spot disease. At the same time, these
results provide a scientific basis for early intervention and
micro-ecological regulation of walnut phyllosphere fungal communities to
prevent and control leaf spot or anthracnose.