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Association of immune responses of Zebu and Holstein-Friesian cattle and resistance to mycobacteria in a BCG challenge model.
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  • Omar Alcaraz-López,
  • Omar Cortéz-Hernández,
  • Guadalupe Vigueras-Meneses,
  • Jacobo Carrisoza-Urbina,
  • Alejandro Benítez-Guzmán,
  • Hugo Esquivel-Solís,
  • Dirk Werling,
  • Francisco Salguero,
  • Hans Vordermeier,
  • Bernardo Villarreal-Ramos,
  • José A. Gutiérrez-Pabello
Omar Alcaraz-López
Universidad Nacional Autonoma de Mexico Facultad de Medicina Veterinaria y Zootecnia
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Omar Cortéz-Hernández
Universidad Nacional Autonoma de Mexico Facultad de Medicina Veterinaria y Zootecnia
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Guadalupe Vigueras-Meneses
Universidad Nacional Autonoma de Mexico Facultad de Medicina Veterinaria y Zootecnia
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Jacobo Carrisoza-Urbina
Universidad Nacional Autonoma de Mexico Facultad de Medicina Veterinaria y Zootecnia
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Alejandro Benítez-Guzmán
Universidad Nacional Autonoma de Mexico Facultad de Medicina Veterinaria y Zootecnia
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Hugo Esquivel-Solís
Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de Jalisco
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Dirk Werling
The Royal Veterinary College_University of London_
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Francisco Salguero
Public Health England Porton
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Hans Vordermeier
Animal and Plant Health Agency
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Bernardo Villarreal-Ramos
Aberystwyth University
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José A. Gutiérrez-Pabello
Universidad Nacional Autonoma de Mexico Facultad de Medicina Veterinaria y Zootecnia
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Abstract

Mycobacterium bovis is the main causative agent of bovine tuberculosis (BTB) in cattle and it is also responsible for a proportion of human TB cases. The annual cost of BTB worldwide is estimated at US$3 billion. Zebu cattle are considered to be more resistant to some infectious diseases than Holstein-Friesian (HF) cattle, including BTB. However, epidemiological studies do not necessarily take into account usage differences of the two types of cattle. It could be argued that HF cattle suffer greater metabolic stress due to their mainly dairy use, whereas Zebu cattle are mainly used for beef production. However, in experiments comparing Zebu and European cattle, the number of animals has been too small to draw statistically robust conclusions on the differences in the level of resistance between these breeds of cattle. Here, we used a recently developed vaccination-and-BCG challenge model to compare the ability of naïve and vaccinated Zebu and HF cattle to control/kill mycobacteria. Young male cattle of both breeds with similar ages were housed in the same accommodation for the duration of the experiment; after correcting for multiple comparison, we found that there was a trend for vaccinated HF cattle to have lower cfu numbers than non-vaccinated HF cattle (ρ = 0.057). No such trend was observed between vaccinated and non-vaccinated Zebu cattle (ρ = 0.560); similarly, no difference was observed between naïve HF and Zebu (ρ = 0.862) cattle. In contrast, evaluation of antigen-specific IFNγ secretion indicated that Zebu and HF cattle differed in their response to mycobacteria. Thus, under the conditions used in this work, the data indicate that there are no differences between Zebu and HF cattle. Further experiments, using larger numbers of animals may be required to determine whether Zebu and HF cattle differ in their susceptibility to infection with M. bovis.

Peer review status:IN REVISION

22 May 2020Submitted to Transboundary and Emerging Diseases
25 May 2020Assigned to Editor
25 May 2020Submission Checks Completed
26 May 2020Reviewer(s) Assigned
01 Jul 2020Review(s) Completed, Editorial Evaluation Pending
24 Jul 2020Editorial Decision: Revise Major