Abstract Background: Nasal polyp is a common disease in otorhinolaryngology, and its clinical diagnosis often needs pathological examination. At present, 3.0T1H-MRS can analyze the spectrum of brain glioma and other diseases, get biochemical metabolism information with clinical diagnosis value, and correctly diagnose brain glioma. Therefore, we use 3.0T1H-MRS to analyze the spectrum of nasal mucosa polyp, in order to make a clear diagnosis of nasal mucosa polyp through 3.0T1H-MRS. Objective： To analyze and study the 3.0T1H-MRS characteristics of nasal polyps, and to explore the value of 3.0T1H-MRS in the diagnosis of nasal polyps. Methods： 50 patients with nasal polyps from January 2010 to June 2019 were randomly selected from our department for study. All patients were pathologically verified and 3.0T was performed MRI, 3.0T1H-MRS focus detection, and quantitative analysis were compared with the normal brain tissue in the same side of the forehead. The main study was acetic acid (Ace), lactic acid (Lac), n-acetylaspartic acid (NAA), choline (Cho), creatine (Cr) peak integral value and the ratio of each integral value: Cho / NAA, Cr / Lac, Cr / NAA, Cho / Cr,Lac / NAA and Lac / Ace. Results：The expression of 3.0T1H-MRS in nasal polyps was significantly different from that in normal brain tissues: NAA decreased, Cho increased,Lac increased, Ace increased and most obvious; Lac / NAA ratio increased; Cho / NAA ratio increased and more obvious; Lac / Ace ratio decreased and most obvious; The difference between the two groups was statistically significant (P < 0.05). Conclusions： The analysis of 3.0T1H-MRS can get the biochemical metabolism information of clinical value: Ace（11.43 ± 6.11）, NAA (17.76 ± 6.20), Cho (23.19 ± 6.91), Lac (12.51 ± 3.42), Lac / Ace (1.09 ± 0.56); Cho / NAA (1.34 ± 1.10), Lac / NAA (0.70 ± 0.55). According to the different integral values of acetic acid, lactic acid, n-acetylaspartic acid, choline and creatine, it is helpful for the correct diagnosis of nasal mucosa polyp.