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SURVEY OF CRIMEAN-CONGO HEMORRHAGIC FEVER VIRUS IN RHIPICEPHALUS AND DERMACENTOR SPECIES TICKS FROM SOUTH-EASTERN ROMANIA
  • +6
  • Bianca Brătuleanu,
  • Delphine Chretien,
  • Béatrice Regnault,
  • Sarah Temmam,
  • Philippe Perot,
  • Adriana Anita,
  • Dragos Anita,
  • Gheorghe Savuta,
  • Marc Eloit
Bianca Brătuleanu
Institut Pasteur
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Delphine Chretien
Institut Pasteur
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Béatrice Regnault
Institut Pasteur
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Sarah Temmam
Institut Pasteur
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Philippe Perot
Institut Pasteur
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Adriana Anita
Universitatea de Stiinte Agricole si Medicina Veterinara Ion Ionescu de la Brad din Iasi
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Dragos Anita
Universitatea de Stiinte Agricole si Medicina Veterinara Ion Ionescu de la Brad din Iasi
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Gheorghe Savuta
Universitatea de Stiinte Agricole si Medicina Veterinara Ion Ionescu de la Brad din Iasi
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Marc Eloit
Institut Pasteur
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Abstract

Few previous studies suggested the circulation of Crimean Congo hemorrhagic fever virus (CCHFV) in Romania, but were based on serological techniques. The present work investigated the presence of CCHFV RNA in Rhipicephalus sp. and Dermacentor sp. ticks, in south-eastern Romania. In July 2019, 127 ticks were collected from the environment and from domestic ruminants (sheep and goats) in five different locations from Tulcea county. All tick samples were tested using Real Time RT-PCR based on SYBR Green with the purpose to detect the CCHFV. For this purpose, specific primer sets for each of the six known CCHFV genotypes and one degenerate primer pair for the detection of all genotypes were used. None of the samples was identified as positive (prevalence <2,5%, p=0.05), highlighting the nil or the low prevalence of CCHFV RNA in Dermacentor sp. and Rhipicephalus sp. ticks from Tulcea county, in 2019. Further studies based on the serological surveillance of animals and more comprehensive ticks testing by molecular biology methods if CCHFV circulates at very low level in Romania.