“Minyak Ala Muncar” or abbreviated as MAM is a by-product waste of
fish canning factory in Muncar Banyuwangi Indonesia. MAM was widely
distributed throughout Indonesia to be used as animal feed supplements.
MAM was very potential to be converted into oil rich in n-3 through the
recycling process. The recycling process needs to be conducted because
MAM contained trans-FA especially EA which could have a detrimental
impact on health. Therefore, the aim of this research was to produce an
oil fraction that rich in n-3 and low in trans-FA content. Three
crystallization methods namely crystallization with n-hexane solvent,
acetone solvent, and urea were chosen as the recycling method. The MAM
Recycling research was carried out through several stages, namely oil
hydrolysis, winterization with solvents (acetone, n-hexane), urea
crystallization, fractions esterification, Fatty Acid content analysis.
The results showed that both crystallization with n-hexane and acetone
produced ratio value of PUFA/trans-FA, n-3/trans-FA, EPA/EA, and DHA/EA
below 0.3 respectively. While urea crystallization was able to produce
ratio numbers for each PUFA/trans-FA, n-3/trans-FA, EPA/EA, and DHA/EA
PUFA/trans-FA 1.46±0.05, 1.36±0.04, 0.73±0.021, 0.48±0.035.