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Biomolecules from Snail mucus (Helix aspersa) conjugate Gold nanoparticles, exhibiting potential wound healing and anti-inflammatory activity
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  • Jennifer Gubitosa,
  • Vito Rizzi,
  • Paola Fini,
  • Anna Laurenzana,
  • Gabriella Fibbi,
  • Clara Veiga-Villauriz,
  • Fiorenza Fanelli,
  • Francesco Fracassi,
  • Alberto Onzo,
  • Giuliana Bianco,
  • Carmine Gaeta,
  • Antonio Guerrieri,
  • Pinalysa Cosma
Jennifer Gubitosa
IPCF-CNR UOS di Bari
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Vito Rizzi
Università degli Studi di Bari Aldo Moro
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Paola Fini
IPCF-CNR UOS di Bari
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Anna Laurenzana
Università degli Studi di Firenze
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Gabriella Fibbi
Università degli Studi di Firenze
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Clara Veiga-Villauriz
The University of Manchester Faculty of Medical and Human Sciences
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Fiorenza Fanelli
Istituto di Nanotecnologia Sede di Bari
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Francesco Fracassi
Università degli Studi di Bari Aldo Moro
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Alberto Onzo
Università degli Studi della Basilicata Dipartimento di Scienze
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Giuliana Bianco
Università degli Studi della Basilicata Dipartimento di Scienze
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Carmine Gaeta
Università degli Studi di Salerno Facoltà di Scienze Matematiche Fisiche e Naturali
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Antonio Guerrieri
Università degli Studi della Basilicata Dipartimento di Scienze
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Pinalysa Cosma
Università degli Studi di Bari Aldo Moro
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Abstract

In this work, for the first time, snail slime from garden snails “Helix Aspersa Müller”, has been used to induce the formation of eco-friendly gold nanoparticles (AuNPs-SS), suitable for biomedical applications. An AuNPs-SS comprehensive investigation was performed observing AuNPs with an average particle size of 14±6 nm, stabilized by a slime snail-based organic layer. Indeed, as recognised in high-resolution MALDI-MS analyses, and corroborated by FESEM, UV-Vis, ATR-FTIR and XPS results, it was possible to assess the main presence of peptides and amino acids as main components of the slime, that, combined with the AuNPs confers them interesting properties. More specifically, we tested, in vitro, the AuNPs-SS safety in human keratinocytes and their potential effect on wound healing as well as their anti-inflammatory properties in Murine Macrophages. Moreover, the AuNPs-SS treatment resulted in a significant increase of the urokinase-type plasminogen activator receptor (uPAR), essential for keratinocyte adhesion, spreading and migration, together with the reduction of LPS-induced IL1-β and IL-6 cytokine levels, and completely abrogated the synthesis of the inducible nitric oxide synthase (iNOS).