Simultaneous detection of lignans and tocopherols in sesame oil by
reversed-phase high-performance liquid chromatography
A rapid and efficient method for the simultaneous detection of lignans
(sesamol, sesamin, sesamolin) and tocopherols (α-tocopherol,
γ-tocopherol, δ-tocopherol) in sesame oil by reversed-phase
high-performance liquid chromatography (RP-HPLC) has been established.
The samples were ultrasonically extracted in methanol and eluted via a
methanol-water gradient on a reversed-phase C18 column with UV/Vis
detection. The recoveries of the six analytes ranged from 88.22% to
100.13%, and the relative standard deviations (RSD, n = 6) ranged from
1.90% to 4.57%. This method allowed for measurement of the
concentrations of lignans and tocopherols in six commercial sesame oils.
This technique is simple, accurate, and highly sensitive and can
therefore be used for the routine analysis of real samples.