Potential of acetamiprid to induce testicular oxidative stress,
apoptosis and hormonal disturbance in male albino Wistar rats
Abstract
Introduction, acetamiprid (AMP) is an insecticide that can affect human
health as it is easily absorbed through gastrointestinal mucosa inducing
erectile dysfunction and reduction of total sperm count. Many research
studies had examined the effect of AMP on different body organs while
this study was designed to study the underlying mechanisms of AMP
associated reproductive toxicity. Material and methods, 40 male albino
Wistar rats were divided into four groups. Control (C) group, received
vehicle. 40 mg dose (40D), 30 mg dose (30D) and 10 mg dose (10D) groups
received 40 mg/kg b.w., 30 mg/kg b.w., 10 mg/kg b.w. of AMP by oral
gavage daily for 90 days. Results, sperm counts were significantly
decreased in AMP treated groups with an increased count of flattened
headed sperms in 40D-group. Plasma and testicular tissue GSH and TAS
levels were significantly decreased while MDA and TOS levels were
significantly increased after AMP administration in a dose dependent
manner while plasma LH, FSH, GnRH and INHB levels were significantly
increased. Seminiferous tubules basement membrane showed irregularities
in 40D and 30D groups with a decrease in spermatogenic cells number. The
testicular proliferative index was decreased associated with an increase
in apoptotic index in AMP treated groups. Conclusion, AMP oral
administration in a dose 10-40 mg/kg b.w. to male albino Wistar rats was
found to possess an oxidative stress and apoptotic impact on the
reproductive system which could be reflected on the occupational health
precautions.