The present study analyzed the characteristics in leaf of Terrestrial Orchidaceae member i.e different species of Habenaria Wild ( H. plantaginea Lind1., H. ovalifolia. Wight., H. multicaudata Sedgw. , H. crinifera Lind1., H. heyneana. Lindl., H. elwesii Hook.f., H. longicorniculata J.Graham. , H. pelorioides Par. & Rchb.f. (Odisha cleistantha S.Mishra).). Anatomical characters are important in identification and ecological adaptations. The anatomical characters have serving as a tool for the genus’s systematic. The plants was collected at different forest types from Shivamogga district. Materials was fixed in FAA solution. Transverse sections were obtained by freehand sections, stained with 1% safranin. Leaf anatomical organization of Habenaria species ( Stomata ,epidermis, mesophyll, vascular bundles, hypodermis.) was observed. There was a distinct variation in the anatomical characters of the leaf. Detailed leaf anatomical characters can be used to distinguish species from others. Leaf anatomical characters with structural adaptation helps plants to survive in different vegetation. The implication of this study is the importance of leaf anatomical features to support species identification and to increase understanding of orchid biology which are important in orchid conservation.