Objectives: The current study was carried out to explore the knowledge, attitude, and practices towards Brucellosis in the people of Khyber Pukhtun Khwa Province, Pakistan. Study design: Cross-sectional study Material and methods: Total of 1600 participants belong to different professions, education level, locality and nature of contact with animals were selected by random sample method. The samples were selected from the Khyber Pakhtun Khwa province of Pakistan. Results: The findings of this study documented poor knowledge regarding brucellosis in the selected participants of current study. Although human and animal health professionals had sufficient knowledge about brucellosis, however no serious trends to control brucellosis were noticed even in health professionals. The unhygienic approach was observed in farmers regarding the handling animal and their products. Self medication approach was also documented in many participants which create antibiotic resistance. Conclusions: Based on the findings we noticed a lack of information regarding zoonotic diseases, health facilities and a weak bond between health and non-health professionals in KPK. Proper education and awareness is highly recommended on zoonotic diseases. Keywords: Zoonotic diseases, Brucellosis, Knowledge, Practices, Attitude