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Tissue Engineered Human Ear Pinna Derived from Decellularized Goat Ear Cartilage: Clinically Useful and Biocompatible auricle construct
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  • Meghnad Joshi,
  • Nilesh Bhamare,
  • Kishor Tardalkar,
  • jeevitaa kshersagar,
  • Shashikant Desai,
  • Tejas Marsale,
  • Mansingraj Nimbalkar,
  • Shimpa Sharma
Meghnad Joshi
D Y Patil Education Society, Deemed to be University
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Nilesh Bhamare
D Y Patil Education Society, Deemed to be University
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Kishor Tardalkar
D Y Patil Education Society, Deemed to be University
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jeevitaa kshersagar
D Y Patil Education Society, Deemed to be University
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Shashikant Desai
Stem Plus Biotech
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Tejas Marsale
D Y Patil Education Society, Deemed to be University
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Mansingraj Nimbalkar
Shivaji University
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Shimpa Sharma
D Y Patil Education Society, Deemed to be University
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Abstract

Surgery of the entire ear pinna even today presents a challenge to reconstructive surgeons, in the absence of a universally acceptable, quality construct for clinical use. In this article, the authors present a technique to generate a flexible, human-size ear with the aim to meet this limitation for ear reconstructive surgeries. The construct was engineered by using a decellularized goat ear cartilage. This was characterized by hematoxylin-eosin (H/E), diamidino-2-phenylindole (DAPI), Masson’s trichrome (MT), Alcian Blue (AB) staining and Scanning Electron Microscopy (SEM) for extracellular matrix (ECM) analysis. The decellularization protocol followed yielded complete removal of all cellular components without changing the properties of the ECM. In vivo biocompatibility of the ear, pinna showed demonstrable recellularization. Recellularization was tracked using HE, DAPI, MT, AB staining, toluidine staining, SEM, vascular-associated protein (VAP), and CD90+ expressing cells. VAP expression revealed specific vasculogenic pattern (angiogenesis). CD90+ expression reflected the presence of the stromal cell. The graft maintained the properties of ECM and displayed chondrocyte recruitment. In summary, the decellularized goat ear pinna (cartilage) exhibited xenograft biocompatibility, stable mechanical properties, and in vivo chondrocyte recruitment. Subsequently developed tissue-engineered ear pinna offer potential for cartilage flexibility and individualization of ear shape and size for clinical application.

Peer review status:POSTED

10 Jul 2020Submitted to Biotechnology and Bioengineering
11 Jul 2020Assigned to Editor
11 Jul 2020Submission Checks Completed