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Role of CBF transcription factors during long-term acclimation to high light and low temperature in two ecotypes of a winter annual
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  • christopher baker,
  • Jared Stewart,
  • Cynthia Amstutz,
  • Jeffrey Johnson,
  • Lindsey Ching,
  • Krishna Niyogi,
  • William W. Adams,
  • Barbara Demmig-Adams
christopher baker
University of California Berkeley
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Jared Stewart
USDA-ARS Plains Area
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Cynthia Amstutz
University of California Berkeley
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Jeffrey Johnson
University of California Berkeley
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Lindsey Ching
University of California Berkeley
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Krishna Niyogi
University of California Berkeley
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William W. Adams
University of Colorado Boulder
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Barbara Demmig-Adams
University of Colorado Boulder
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Abstract

When grown under cool temperature, winter annuals respond with not only enhanced freezing tolerance but also photosynthetic upregulation. The role of the cold-induced C-repeat-Binding Factor (CBFs) in long-term maintenance of freezing tolerance and photosynthetic upregulation was examined in two Arabidopsis thaliana ecotypes adapted to differing climates (Italy = IT and Sweden = SW) as well as corresponding CBF-disabled mutant lines. Data on photosynthetic, morphological, and freezing-tolerance phenotypes as well as transcriptomic data were collected from plants grown for several weeks under controlled conditions with several combinations of temperature and light levels. Freezing tolerance in these acclimated plants depended strongly on CBFs in both SW and IT. In contrast, photosynthetic upregulation was the same, or modestly reduced, in cbf mutant versus parental lines of SW and IT, respectively. Physiological and transcriptomic data showed a consistent trend for a greater role of CBFs in cool-temperature-grown plants of IT versus SW. These features suggest that IT remained in a state of continuing CBF-related cold-acclimation even after weeks of acclimation, while SW entered a state of completed acclimation in which maintenance of photosynthetic upregulation no longer required CBF activation and maintenance of freezing tolerance was less dependent on CBF than in IT.

Peer review status:UNDER REVIEW

17 Jul 2020Submitted to Plant, Cell & Environment
18 Jul 2020Assigned to Editor
18 Jul 2020Submission Checks Completed
21 Jul 2020Reviewer(s) Assigned