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Metabolic flux from the chloroplast provides signals controlling photosynthetic acclimation to cold in Arabidopsis thaliana.
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  • Helena Herrmann,
  • Beth Dyson,
  • Matthew Miller,
  • Jean Marc Schwartz,
  • Giles Johnson
Helena Herrmann
University of Manchester
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Beth Dyson
University of Sheffield
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Matthew Miller
University of Manchester
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Jean Marc Schwartz
University of Manchester
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Giles Johnson
University of Manchester
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Abstract

Photosynthesis is especially sensitive to environmental conditions and the composition of the photosynthetic apparatus can be modulated in response to environmental change, a process termed photosynthetic acclimation. Previously, we identified a role for a cytosolic fumarase, FUM2 in acclimation to low temperature in Arabidopsis thaliana. Mutant lines lacking FUM2 were unable to acclimate their photosynthetic apparatus to cold. Here, using gas exchange measurements and metabolite assays of acclimating and non-acclimating plants, we show that acclimation to low temperature results in a change in the distribution of photosynthetically fixed carbon to different storage pools during the day. Proteomic analysis of wild-type Col-0 Arabidopsis and of a fum2 mutant which was unable to acclimate to cold indicates that extensive changes occurring in response to cold are affected in the mutant. Metabolic and proteomic data were used to parameterise metabolic models. Using an approach called flux sampling, we show how the relative export of triose phosphate and 3-phsphoglycerate provides a signal of the chloroplast redox state that could underly photosynthetic acclimation to cold.

Peer review status:Published

20 Jul 2020Submitted to Plant, Cell & Environment
21 Jul 2020Submission Checks Completed
21 Jul 2020Assigned to Editor
24 Jul 2020Reviewer(s) Assigned
12 Aug 2020Review(s) Completed, Editorial Evaluation Pending
08 Sep 20201st Revision Received
08 Sep 2020Submission Checks Completed
08 Sep 2020Assigned to Editor
09 Sep 2020Review(s) Completed, Editorial Evaluation Pending
27 Sep 2020Published in Plant, Cell & Environment. 10.1111/pce.13896