loading page

Genetic characterization of rabies virus circulating in crab-eating fox (Cerdocyum thous) in the State of Paraiba, Northeastern Brazil
  • +9
  • Camila Bezerra,
  • Albério Gomes,
  • Sérgio Santos de Azevedo,
  • Clebert Alves,
  • Leila Ullmann,
  • Camila Malossi,
  • João Pessoa,
  • Tiago Lopes,
  • Mauricio Nogueira,
  • Jeann Araújo,
  • Antônio Dantas,
  • Luana Silva
Camila Bezerra
Federal University of Campina Grande
Author Profile
Albério Gomes
Federal University of Campina Grande
Author Profile
Sérgio Santos de Azevedo
Universidade Federal de Campina Grande
Author Profile
Clebert Alves
Universidade Federal de Campina Grande
Author Profile
Leila Ullmann
Universidade Estadual Paulista
Author Profile
Camila Malossi
UNESP
Author Profile
João Pessoa
IBB-UNESP
Author Profile
Tiago Lopes
The University of Tokyo Institute of Medical Science
Author Profile
Mauricio Nogueira
FAMERP
Author Profile
Jeann Araújo
UFPB
Author Profile
Antônio Dantas
UFCG
Author Profile
Luana Silva
Federal University of Campina Grande
Author Profile

Abstract

Rabies is a viral zoonotic disease present in two thirds of all countries, and causes the death of one person every 10 minutes (~70,000 deaths/year). The reservoirs of the Rabies Virus (RABV) are bats and canids, and it has also been found in other animals, including Cerdocyon thous (crab-eating fox) and Pseudalopex vetulus (hoary fox). Here we used Next Generation Sequencing (NGS), phylogenetic, and in vitro/in vivo analyses, to characterized the genome of a new subtype of RABV circulating in foxes of the Northeastern region of Brazil. We verified that although these variants were similar to existing strains from wild canids and domestic canines from Brazil, the samples contained escape mutants, suggesting that it was a heterogeneous virus population. In all, we used several molecular techniques to characterize a new RABV strain circulating in wild-foxes in Northeastern Brazil, and verified still manifested its notorious pathogenic characteristics.