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Improved cultivation of CHO cells in bioreactor with reciprocal mixing
  • +3
  • Masashi UEKI,
  • Noriyuki Tansho,
  • Makoto Sato,
  • Hisayuki Kanamori,
  • Yoshihiro Ito,
  • Yoshikazu Kato
Masashi UEKI
RIKEN
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Noriyuki Tansho
Satake Chemical Equipment Mfg., Ltd., Japan
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Makoto Sato
Satake Chemical Equipment Mfg., Ltd., Japan
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Hisayuki Kanamori
Satake Chemical Equipment Mfg., Ltd., Japan
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Yoshihiro Ito
RIKEN
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Yoshikazu Kato
Satake Chemical Equipment Mfg., Ltd., Japan
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Abstract

We have constructed a new bioreactor with reciprocal mixing that is better suited for the cultivation of delicate animal cells. In-silico simulation (computational fluid dynamics) suggested both maximum and average shear stresses in the bioreactor with reciprocal mixing to be remarkably lower than in conventional bioreactor with rotary mixing. Although we could not find any difference in growth speed and cell density between the bioreactors with reciprocal and rotary mixing, we did find cell viability in reciprocal-mixing bioreactor to be retained longer than in rotary-paddle bioreactor. This implied that cell culture in a bioreactor with reciprocal mixing could be prolonged for the production of target proteins. Leakage of lactate dehydrogenase activity into the culture medium was suppressed much more in the reciprocal-mixing bioreactor than in the rotary-paddle one. Production of human tissue plasminogen activator in the former was also observed to be much more than in the latter. Therefore, bioreactor with reciprocal mixing was concluded to be better suited for the cultivation of animal cells and efficient production of proteins, such as antibody drugs and various growth factors.

Peer review status:UNDER REVIEW

29 Jul 2020Submitted to Biotechnology and Bioengineering
29 Jul 2020Assigned to Editor
29 Jul 2020Submission Checks Completed
03 Sep 2020Reviewer(s) Assigned