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DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS THROUGH THE NATURAL MATRIX TO NEUROSPHERES FOR CHOLINERGIC-LIKE CELLS
  • +8
  • Katherine Carvalho,
  • Priscila Elias Ferreira Stricker,
  • Daiany de Souza,
  • Ana Carolina Irioda,
  • Bassam Felipe Mogharbel,
  • Celia Regina Cavichiolo Franco,
  • José Roberto de Souza Almeida Leitec,,
  • Alyne Rodrigues de Araújo,
  • Felipe Azevedo Borgese,,
  • Rondinelli Donizetti Herculano,
  • Frederico de Oliveira Graefff
Katherine Carvalho
Author Profile
Priscila Elias Ferreira Stricker
Daiany de Souza
Ana Carolina Irioda
Bassam Felipe Mogharbel
Celia Regina Cavichiolo Franco
José Roberto de Souza Almeida Leitec,
Alyne Rodrigues de Araújo
Felipe Azevedo Borgese,
Rondinelli Donizetti Herculano
Frederico de Oliveira Graefff

Abstract

This study aimed to differentiate human mesenchymal stem cells (hMSCs) from the human umbilical cord in cholinergic-like cells using a natural matrix. The isolation of hMSCs from Wharton's jelly (WJ) was carried out using "explant" and mononuclear cells by density gradient. hMSCs were plated in a natural functional biopolymer matrix for the production of neurospheres. Neural precursor cells were subjected to a standard cholinergic differentiation protocol. Dissociated neurospheres, neural precursor cells, and cholinergic-like cells were characterized by immunocytochemistry. The RT-PCR was performed. hMSCs were CD73+, CD90+, CD105+, CD34- and CD45- and demonstrated the trilineage differentiation. Neurospheres and their isolated cells were nestin-positive, and also expressed NESTIN, MAP2, ßIII-TUBULIN, GFAP genes. Neural precursor cells that were differentiated in cholinergic-like cells expressed ßIII-TUBULIN protein and choline acetyltransferase enzyme. hMSCs on the natural matrix were capable of differentiating hMSC into neurospheres, obtaining neural precursor cells without growth factors or gene transfection before cholinergic differentiation.