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Predominance of Extreme Environmental Conditions at High Altitude in Modulating Human Gut Microbiota
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  • Brij Bhushan,
  • Malleswara Eslavath,
  • Anand Yadav,
  • Ashish Srivastava,
  • Maran Prasanna Reddy,
  • Tsering Norboo,
  • Bhuvnesh Kumar,
  • Lilly Ganju,
  • Shashibala Singh
Brij Bhushan
DRDO Defence Institute of Physiology and Allied Sciences
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Malleswara Eslavath
DRDO Defence Institute of Physiology and Allied Sciences
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Anand Yadav
DRDO Defence Institute of Physiology and Allied Sciences
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Ashish Srivastava
DRDO Defence Institute of Physiology and Allied Sciences
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Maran Prasanna Reddy
DRDO Defence Institute of Physiology and Allied Sciences
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Tsering Norboo
Ladakh Institute for Prevention (LIP)
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Bhuvnesh Kumar
DRDO Defence Institute of Physiology and Allied Sciences
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Lilly Ganju
DRDO Defence Institute of Physiology and Allied Sciences
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Shashibala Singh
National Institute of Pharmaceuticals Education and Research (NIPER)
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Abstract

Human microbial alterations are associated with environmental stress, nutritional, genetic and triggering de-novo variations. Nevertheless, human gut microbiome at extreme altitude (>5800 m) remains unexplored. We aimed to demonstrate the microbial predominance in individuals with same ethnicity and dietary pattern at extreme altitude with unique challenges like cold, hypoxia, radiation etc. Different analysis pipelines were used for fecal whole genome sequencing at 210m, 3500m, 4420m and 5805m, and 16s rRNA V3-V4 regions amplification sequencing of 19 individuals belonging to the same ethnicity and dietary pattern, for presence of taxonomy & functional potential and confirming the prediction upto the strain level within the same cohort. Principal component analysis, revealed distinct microbiome changes at different altitudes, with varied and higher Bacteroides and Prevotella ratio. There was predominance of genus Prevotella at altitudes 4420m & 5805m than at 210m & 3500m. Appearance of species Prevotella copri strain 61740 was increasing significantly at extreme altitudes, whereas co-occurrence of other bacterial strains had different pattern than Prevotella. The extensive strain level analysis indicated alteration in the metabolic pathways. This study under stressful and hypoxic environment of extreme altitudes, associated microbial variation with altered metabolic pathways, reveals influence of extreme environment on human gut microbiota with predominance of Prevotella.

Peer review status:POSTED

03 Aug 2020Submitted to Biotechnology and Bioengineering
03 Aug 2020Assigned to Editor
03 Aug 2020Submission Checks Completed