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Chromosome-level genome of the peach fruit moth Carposina sasakii (Lepidoptera: Carposinidae) provides a resource for evolutionary studies on moths
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  • Li-Jun Cao,
  • Wei Song,
  • Lei Yue,
  • Shao-Kun Guo,
  • Jin-Cui Chen,
  • Ya-Jun Gong,
  • Ary Hoffmann,
  • Shu-Jun Wei
Li-Jun Cao
Beijing Academy of Agriculture and Forestry Sciences
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Wei Song
Beijing Academy of Agriculture and Forestry Sciences
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Lei Yue
Beijing Academy of Agriculture and Forestry Sciences, Beijing Academy of Agriculture and Forestry Sciences
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Shao-Kun Guo
Beijing Academy of Agriculture and Forestry Sciences
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Jin-Cui Chen
Beijing Academy of Agriculture and Forestry Sciences, Beijing Academy of Agriculture and Forestry Sciences
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Ya-Jun Gong
Beijing Academy of Agriculture and Forestry Sciences
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Ary Hoffmann
The University of Melbourne Bio21 Molecular Science and Biotechnology Institute
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Shu-Jun Wei
Beijing Academy of Agriculture and Forestry Sciences
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Abstract

The peach fruit moth (PFM), Carposina sasakii Matsumura, is a major phytophagous orchard pest widely distributed across Northeast Asia. Here, we report the chromosome-level genome for the PFM, representing the first genome for the family Carposinidae, from the lepidopteran superfamily Copromorphoidea. The genome was assembled into 404.83 Mb sequences using PacBio long-read and Illumina short-read sequences, including 275 contigs, with a contig N50 length of 2.62 Mb. All contigs were assembled into 32 linkage groups assisted by the Hi-C technique, including 30 autosomes, a female specific W chromosome and a Z chromosome. BUSCO analysis showed that 98.2% genes were complete and 0.4% of genes were fragmented, while 1.4% of genes were missing in the assembled genome. In total, 23,218 protein-coding genes were predicted, of which 82.72% were functionally annotated. Because of the importance of diapause triggered by photoperiod in PFM, five circadian genes in the PFM as well as in the other related species were annotated, and potential genes related to diapause and photoperiodic reaction were also identified from transcriptome sequencing. In addition, manual annotation of detoxification gene families was undertaken and showed a higher number of ABC and GST genes in PFM than in most other lepidopterans, in contrast to a lower number of UGT genes, suggesting different detoxication pathways in this moth. The high-quality genome provides a resource for comparative evolutionary studies of this moth and its relatives within the context of radiations across Lepidoptera.

Peer review status:UNDER REVIEW

29 Jul 2020Submitted to Molecular Ecology Resources
14 Aug 2020Assigned to Editor
14 Aug 2020Submission Checks Completed
17 Aug 2020Reviewer(s) Assigned
28 Aug 2020Review(s) Completed, Editorial Evaluation Pending
14 Sep 2020Editorial Decision: Revise Minor
23 Sep 20201st Revision Received
23 Sep 2020Review(s) Completed, Editorial Evaluation Pending