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5'-Ribonucleotides production using 5'-phosphodiesterase from spent malt roots
  • Edson Marcelino Alves,
  • JOyce Faria de Souza,
  • Pedro de Oliva Neto
Edson Marcelino Alves
Universidade Estadual Paulista Julio de Mesquita Filho
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JOyce Faria de Souza
Universidade Estadual Paulista Julio de Mesquita Filho
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Pedro de Oliva Neto
Universidade Estadual Paulista Julio de Mesquita Filho
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Abstract

The present work aims to produce a composition of 5’-ribonucleotides using spent brewer’s yeast as cheap source of RNA and barley malt rootlets as cheap source of 5’-phosphodiesterase (5’-PDE). This strategy is very promising because both are residues of the brewing process and are closely linked in a cycle that until now is not yet commercially exploited due to lack of studies. The results of the present work showed that extraction of 5’-PDE was mainly influenced by the fineness of the rootlets and amount of extraction solvent (water). The main molecules formed during RNA hydrolysis were 5’-ribonucleotides, which represented 85.86% of the total hydrolyzed molecules. Finally, the results of the approach here proposed can generate a new perspective for the brewing industry in relation to the management of its wastes in order to generate from them products of high added value and with a wide range of applications.

Peer review status:POSTED

19 Aug 2020Submitted to Biotechnology and Bioengineering
20 Aug 2020Assigned to Editor
20 Aug 2020Submission Checks Completed