Objectives: To compare levels of catecholaminergic system members, renalase, cerebellin, and their subtrates, epinephrine, norepinephrine and dopamine and apelinergic system members, apelin, elabela and nitric oxide in the blood and urine of patients with acute ischemic stroke and healthy controls. Materials and Methods: 42 patients with acute ischemic stroke and 42 age and sex matched healthy controls were included. Patients had first ischemic stroke attack and aged older than 18 years old. Blood and urine samples were collected simultaneously and within first 24 hours after the onset of acute stroke clinical manifestations and were measured using enzyme-linked immunosorbent assay method. Results: The levels of serum and urine cerebellin, renalase, epinephrine, norperinephrine, dopamine, apelin, elebela and nitric oxide were similar in ischemic stroke and in control groups (P> 0.05). Strong correlations were found between renalase, cerebellin and catecholamine levels in serum and urine (p <0.001) both in stroke patients and controls. There were also strong correlations between apelin, elabela and NO levels in serum and urine (p <0.001) in two groups. Conclusions: Serum and urine cerebellin, renalase, epinephrine, norperinephrine, dopamine, apelin, elebela and nitric oxide levels do not significantly change in the acute phase of ischemic stroke. Strong correlations among renalase, cerebellin and catecholamines emphasize that these substances act together in healthy individuals and ischemic stroke patients. Similarly, strong correlations between apelin, elabela and NO indicate that these agents act together in healthy subjects and patients with ischemic stroke.