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Development of a novel real-time PCR assay for rapid detection of African swine fever virus (ASFV) strains circulating in Vietnam
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  • Thi Bich Ngoc Trinh,
  • Thang Truong,
  • Tam Nguyen,
  • Xuan Dang Vu,
  • Anh Dao,
  • Thi Lan Nguyen,
  • Aruna Ambagala,
  • Shawn Babiuk,
  • Jinsik Oh,
  • Daesub Song,
  • Van Phan Le
Thi Bich Ngoc Trinh
Vietnam National University of Agriculture
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Thang Truong
Public Health Agency of Canada Ontario Manitoba Saskatchewan Regional Office
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Tam Nguyen
Vietnam National University of Agriculture
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Xuan Dang Vu
Vietnam National University of Agriculture
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Anh Dao
Vietnam National University of Agriculture
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Thi Lan Nguyen
Vietnam National University of Agriculture
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Aruna Ambagala
Canadian Food Inspection Agency
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Shawn Babiuk
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Jinsik Oh
Median Diagnostics
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Daesub Song
Korea University
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Van Phan Le
College of Veterinary Medicine. Vietnam National University of Agriculture, Vietnam
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Abstract

African swine fever (ASF) continues to cause outbreaks throughout regions of Africa, Europe and Asia. The disease can cause severe morbidity and mortality resulting in serious economic losses. Since there is no vaccine available to control ASF, early detection is critical to contain and control the disease. The aim of this study was to develop a novel real-time PCR assay based on highly conserved ASFV gene E183L (p54). The limit of detection of the assay, VNUA-54 real-time PCR, was 2.63 copies/reaction and 2 Log10 HAD50/ml. The VNUA-54 real-time PCR was able to detect fifteen different ASFV reference strains representing p72 genotypes I, II and V. The assay was specific and did not amplify other swine viruses including CSF, FMD, PRRS, and PED. The diagnostic sensitivity of the real-time PCR assay was evaluated using 187 field clinical specimens collected from swine farms located in different provinces in Vietnam. The VNUA-54 real-time PCR assay is an additional tool for ASF diagnostics and can be used in combination with other p72 based ASFV real-time PCR assays as a rapid confirmatory assay.