loading page

Activated steady status and distinctive FcεRI-mediated responsiveness in basophils of atopic dermatitis
  • +3
  • Shinya Imamura,
  • Ken Washio,
  • Mayuko Mizuno,
  • Yoshiko Oda,
  • Atsushi Fukunaga,
  • Chikako Nishigori
Shinya Imamura
Kobe University Graduate School of Medicine School of Medicine Dermatology

Corresponding Author:[email protected]

Author Profile
Ken Washio
Kobe University Graduate School of Medicine School of Medicine Dermatology
Author Profile
Mayuko Mizuno
Kobe University Graduate School of Medicine School of Medicine Dermatology
Author Profile
Yoshiko Oda
Kobe University Graduate School of Medicine School of Medicine Dermatology
Author Profile
Atsushi Fukunaga
Kobe University Graduate School of Medicine School of Medicine Dermatology
Author Profile
Chikako Nishigori
Kobe University Graduate School of Medicine School of Medicine Dermatology
Author Profile

Abstract

BACKGROUND: Although basophils are considered to play an important role for maintenance of type 2 inflammation in atopic dermatitis (AD), studies on basophils in AD patients are limited. Some studies have reported the activation status, including CD203c and CD63, of peripheral blood basophils in AD patients. OBJECTIVE: To examine the features of circulating basophils in AD patients by assessing and comparing cell surface marker expressions, total serum IgE, and basophil responsiveness to stimulation between AD patients and healthy controls (HCs). In addition, to examine the correlations among AD severity, laboratory factors, and features of basophils. METHODS: Blood samples from 38 AD patients and 21 HCs were analyzed. Basophil response markers CD203c and CD63, and expression of surface-bound IgE and FcεRI on basophils were measured. CD203c and CD63 expressions induced by stimulation with anti-IgE and anti-FcεRI antibodies were measured. Clinical/laboratory factors including total serum IgE were examined for correlations with these basophil parameters. RESULTS: Basophils in AD patients were activated spontaneously without stimulation and exhibited low responsiveness to anti-IgE antibody stimulation. Responsiveness to anti-FcεRI stimulation was higher than anti-IgE stimulation, contrary to HCs. FcεRI expression on basophils was higher in AD patients than in HCs, although surface-bound IgE on basophils was equivalent. Total serum IgE had negative correlations with surface-bound IgE and CD63 responsiveness to anti-IgE stimulation. CONCLUSIONS: Our findings illustrate the characteristic basophil status in AD. Despite high serum IgE and high basophil FcεRI expression, surface-bound IgE on basophils remained relatively low and basophils were spontaneously activated under steady-state conditions.
Mar 2021Published in Allergology International. 10.1016/j.alit.2021.01.005