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Multi-Enzyme Cascade Reaction in a Miniplant Two-Phase-System: Model Validation and Mathematical Optimization
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  • Jens Johannsen,
  • Francesca Meyer,
  • Claudia Engelmann,
  • Andreas Liese,
  • Georg Fieg,
  • Paul Bubenheim,
  • Thomas Waluga
Jens Johannsen
Hamburg University of Technology
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Francesca Meyer
Hamburg University of Technology
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Claudia Engelmann
Hamburg University of Technology
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Andreas Liese
Hamburg University of Technology
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Georg Fieg
Hamburg University of Technology
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Paul Bubenheim
Hamburg University of Technology
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Thomas Waluga
Hamburg University of Technology
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Abstract

Biotechnological application of multiple enzymes in different phases for target compounds synthesis poses a significant challenge for industrial process development. At the same time, a growing demand for natural flavors and fragrances opens up possibilities for novel biotechnological processes to replace current chemical synthesis routes, with additional advantages such as avoiding harsh reaction conditions and toxic chemicals, and less by-products in the system. Within complex biotechnological processes, the key for unfolding their industrial application potential in bioprocess engineering lies in their mathematical modeling. In this contribution, a multi-enzyme cascade reaction in a two-phase system implemented in a miniplant-scale reactor setup is mathematically modeled for the example of the flavoring agent cinnamyl cinnamate. Using our validated model and a mathematical optimization tool based on a genetic algorithm, optimization runs are performed to demonstrate the potential of computer-aided process development for complex biotechnological processes.

Peer review status:UNDER REVIEW

11 Aug 2020Submitted to AIChE Journal
28 Aug 2020Assigned to Editor
28 Aug 2020Submission Checks Completed
04 Sep 2020Reviewer(s) Assigned