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Bio-functionalized titanium surfaces with the modified silk fibroin carrying titanium binding motif enhanced the ossific differentiation of MC3T3-E1
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  • Mai Watanabe,
  • Ujjal Bhawala,
  • Shinji Takemoto,
  • Norihiro Nishiyama,
  • Yuichi Nakahara,
  • Ken-ichiro Tatematsu,
  • Hideki Sezutsu,
  • Nobuo Kuwabara,
  • Tamiko Minamisawa,
  • Kiyotaka Shiba,
  • Tetsuo Asakura
Mai Watanabe
Public Interest Incorporated Foundation Japanese Foundation for Cancer Research
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Ujjal Bhawala
Nihon University School of Dentistry Graduate School of Dentistry at Matsudo
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Shinji Takemoto
Iwate Medical University
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Norihiro Nishiyama
Nihon University School of Dentistry Graduate School of Dentistry at Matsudo
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Yuichi Nakahara
National Agriculture and Food Research Organization
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Ken-ichiro Tatematsu
National Agriculture and Food Research Organization
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Hideki Sezutsu
National Agriculture and Food Research Organization
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Nobuo Kuwabara
Gunma Sericultural Technology Center
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Tamiko Minamisawa
Public Interest Incorporated Foundation Japanese Foundation for Cancer Research
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Kiyotaka Shiba
Public Interest Incorporated Foundation Japanese Foundation for Cancer Research
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Tetsuo Asakura
Tokyo University of Agriculture and Technology
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Abstract

Silk fibroin (SF) from Bombyx mori has superior properties as both a textile and a biomaterial, and has been used to functionalize the surfaces of various medical inorganic materials including titanium (Ti). In this paper, we endowed SF with reversible binding ability to Ti by embedding a titanium binding motif (minTBP-1, RKLPDA). Artificial SF proteins were first created by conjugating gene cassettes for SF motif (AGSGAG) and minTBP-1 motif with different ratios, which have been shown to bind reversibly to Ti surfaces in quartz crystal microbalance analyses. Based on these results, the functionalized SF (TiBP-SF) containing the designed peptide [TS[(AGSGAG)3AS]2RKLPDAS]8 was prepared from the cocoon of transgenic B. mori, which accelerates the ossific differentiation of MC3T3-E1 cells when coated on titanium substrates. Thus, TiBP-SF presents an alternative for endowing the surfaces of titanium materials with osseointegration functionality, which would allow the exploration of potential applications in the medical field.

Peer review status:POSTED

31 Aug 2020Submitted to Biotechnology and Bioengineering
31 Aug 2020Assigned to Editor
31 Aug 2020Submission Checks Completed