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A trans-dihydrocarvone-producing bacterial strain Klebsiella sp. O852 with potential use in bio-flavor production
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  • Lulu Zhang,
  • Jing-Nan Ren,
  • Gang Fan,
  • Jin He,
  • Wen Huang,
  • Ying-Ying Zhang,
  • Zhi Li,
  • Xiao Li,
  • Si-Yi Pan
Lulu Zhang
Huazhong Agriculture University
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Jing-Nan Ren
Huazhong Agriculture University
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Gang Fan
Huazhong Agricultural University
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Jin He
Huazhong Agricultural University
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Wen Huang
Huazhong Agriculture University
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Ying-Ying Zhang
Huazhong Agriculture University
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Zhi Li
Huazhong Agriculture University
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Xiao Li
Huazhong Agriculture University
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Si-Yi Pan
Huazhong Agriculture University
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Abstract

Celery spices are an important raw material in the production of food and cosmetic, including carveol, carvone and dihydrocarvone. In this study, a new Klebsiella sp. O852 was isolated, which was capable of converting limonene to trans-dihydrocarvone. The yields of trans-dihydrocarvone reached up to 1058 mg/L when the strain O852 was incubated using LB-M medium for 4 h at 36ºC and 150 rpm and the process was monitored for 36 h after adding 1680 mg/L limonene dissolved in ethanol. Limonene was used as a 20% (v/v) solution in the ethanol. Besides, the genome of Klebsiella sp. O852 comprised 20 contigs and 19 scaffolds. The genome size was 5.49 Mb. A total of 5218 protein-encoding genes were predicted, these genes mainly distributed in some metabolism and biosynthesis categories. Finally, several genes involved in trans-dihydrocarvone biosynthesis were further analyzed, identified and verified by quantitative real-time polymerase chain reaction (RT-qPCR) and exogenous expression. These novel genes may find value in the bioconversion of inexpensive raw materials to natural flavors and fragrances.

Peer review status:POSTED

09 Sep 2020Submitted to Biotechnology and Bioengineering
09 Sep 2020Assigned to Editor
09 Sep 2020Submission Checks Completed