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Improvement of Xylose Utilization and L-ornithine Production by Metabolic Engineering of Corynebacterium glutamicum
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  • Ge Gao,
  • Yan Zhang,
  • Ying Zhou,
  • Bang-Ce Ye
Ge Gao
East China University of Science and Technology
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Yan Zhang
Shihezi University
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Ying Zhou
East China University of Science and Technology
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Bang-Ce Ye
State Key Laboratory of Bioreactor Engineering
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Abstract

L-ornithine is a basic amino acid, which shows significant value in food and medicine industries. There is a huge space for L-ornithine production with strains available for metabolic engineering, and it is urgent to develop a high-efficiency engineering strain for industrialization. Here, xylose isomerase and xylulose kinase were introduced into Corynebacterium glutamicum S9114 to establish xylose metabolism pathway, and then xylose became a substitute carbon source of glucose. In addition, the optimization and overexpression of phosphoenolpyruvate carboxylase and pentose transporter have been conducted to promote the synthesis of L-ornithine for the first time. Furthermore, though optimizing the concentration ratio of glucose and xylose (7:3), adding biotin and thiamine hydrochloride, we arrived at the highest L-ornithine yield 41.5g/L in shaking flask fermentation so far. Our results demonstrate that the combination of metabolic engineering and the optimization of fermentation process can make great potential for L-ornithine production by lignocellulose hydrolysate.

Peer review status:UNDER REVIEW

03 Sep 2020Submitted to Biotechnology and Bioengineering
03 Sep 2020Assigned to Editor
03 Sep 2020Submission Checks Completed
08 Sep 2020Review(s) Completed, Editorial Evaluation Pending