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NOVOWrap: An automated solution for plastid genome assembly and structure standardization
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  • Ping Wu,
  • Hao Chen,
  • Chao Xu,
  • Jie Yang,
  • Xian-chun Zhang,
  • Shi-Liang Zhou
Ping Wu
Institute of Botany Chinese Academy of Sciences
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Hao Chen
Shaanxi University of Science and Technology
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Chao Xu
Institute of Botany Chinese Academy of Sciences
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Jie Yang
Institute of Botany Chinese Academy of Sciences
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Xian-chun Zhang
Institute of Botany Chinese Academy of Sciences
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Shi-Liang Zhou
Institute of Botany Chinese Academy of Sciences
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Abstract

Plastid genomes are unique to plants and play an important role in genomics and evolutionary biology. Next-generation sequencing has revolutionized plastid genome data acquisition in a way that genome assembly and annotation became bottlenecks for large plastid genome data usage. Here we develop a novel open-source, cross-platform tool, NOVOWrap, with both command-line and graphical user interfaces for plastid genome automatic assembly using personal computers. With minimum inputs and user intervention, NOVOWrap could automatically assemble plastid genomes, validate results and standardize the structure with affordable computer resources. The performance of the software has been successfully benchmarked against eleven plastid genomes of species belonging to lycopods, gymnosperms, and angiosperms. The program is expected to liberate researchers from laborious computer manipulations and create reliable and standard genomic data.

Peer review status:UNDER REVIEW

27 Aug 2020Submitted to Molecular Ecology Resources
08 Sep 2020Assigned to Editor
08 Sep 2020Submission Checks Completed
09 Sep 2020Reviewer(s) Assigned