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Physiological and Molecular Characterization of Bread Wheat (Triticum aestivum L.) For Drought Resistance
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  • Ahmad Zada,
  • Ahmed Ali,
  • Azhar Shah,
  • Shahid Gill,
  • Irtiza Hussain,
  • Zahid Ullah,
  • Hassan Sher
Ahmad Zada
Yangzhou University
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Ahmed Ali
University of Swat
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Azhar Shah
Hazara University Mansehra
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Shahid Gill
PARC
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Irtiza Hussain
Arid Agriculture University of Rawalpindi
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Zahid Ullah
University of Swat
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Hassan Sher
University of Swat
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Abstract

The present study evaluated three wheat genotypes (SD-28, SD-32 and Chirya-1) were evaluated for physiological attributes like Relative Water Content, Proline Content, Membrane Stability Index and Chlorophyll Content where Opata was used as a control check under three different levels of drought stress (100% FC, 80% FC and 60% FC). Results revealed that chlorophyll content was significantly affected under stressed conditions in all the studied genotypes and genotypes. Molecular diagnosis of the selected wheat genotypes and genotypes was carried out with RT-PCR using expression profile of 06 genes (TaLhca1, TaLhca2, TaLhca3, TaLhcb1, TaLhcb4 and TaLhcb6) that encodes for LHCI and LHCII proteins. RT–PCR indicated variable expression of the selected genes in response to different level of drought stress. The results obtained clearly showed the relation between genotypes and severity of drought stress condition. Among the studied genotypes Chirya-1 and SD-28 performed well with higher level of gene expression under drought stress condition; and may be considered drought tolerant genotypes with potential to enrich the genetic background of locally adapted wheat lines against drought stress.